Sandrine Holt made her feature film debut in Bruce Beresford's epic Black Robe (1991), which earned her a Genie Award nomination for Best Supporting Actress. She has starred in such films as Rapa Nui (1994), Dance Me Outside (1994) and Pocahontas: The Legend (1995). In addition, she co-starred with Jennifer Garner, Buck Henry and Amanda Peet in 1999 (1998), Giovanni Veronesi's Il mio West (1998) starring David Bowie and Harvey Keitel, Loving Jezebel (1999), Fast Food Fast Women (2000), The Mission (1994) and Century Hotel (2001). Most recently she appeared in Resident Evil: Apocalypse (2004) with Milla Jovovich and Oded Fehr, and Happy Hour (2003) with Anthony LaPaglia and Eric Stoltz. Her television credits include the lead role in the John Woo series Once a Thief (1996) and guest appearances on New York Undercover (1994).
When she was 5, she moved to Toronto, Canada, with her Chinese father and French mother.